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Original Article
 

Expression of Inducible Nitric Oxide Synthase in a Mouse Model of Anaphylaxis
 

K Sade,1,2 IF Schwartz,2,3 S Etkin,1 S Schwartzenberg,2 Y Levo,2 S Kivity1

1 The Allergy & Asthma Center, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
2 Department of Medicine ‘T’, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
3 Department of Nephrology, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel

J Investig Allergol Clin Immunol 2007; Vol. 17(6): 379-385
 

 Abstract


Background: The generation of large quantities of nitric oxide (NO) is implicated in the pathogenesis of anaphylactic shock. The source of NO, however, has not been established and conflicting results have been obtained when investigators have tried to inhibit its production in anaphylaxis.

Objective: The aim of this study was to analyze the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in a mouse model of anaphylaxis.

Methods: BALB/c mice were sensitized and challenged with ovalbumin to induce anaphylaxis. Tissues were removed from the heart and lungs, and blood was drawn at different time points during the fi rst 48 hours after induction of anaphylaxis. The Griess assay was used to measure nitric oxide generation. Nitric oxide synthase expression was examined by reverse transcriptase polymerase chain reaction and immunohistochemistry.

Results: A signifi cant increase in iNOS mRNA expression and nitric oxide production was evident as early as 10 to 30 minutes after allergen challenge in both heart and lungs. In contrast, expression of eNOS mRNA was not altered during the course of the experiment.

Conclusion: Our results support involvement of iNOS in the immediate physiological response of anaphylaxis.

Key words: Anaphylaxis. Shock. Allergy. Nitric oxide. Inducible nitric oxide synthase